Mod Pack Studio 2.0 runs with all versions of Windows*. However, you may receive an error message, "16-bit MS-DOS Subsystem...The system file is not suitable for running MS-DOS and Microsoft Windows applications..."
Reason, one or more of the following files are missing and/or damaged on your computer, autoexec.nt, command.com, and config.nt.
This is NOT a Windows or Mod Pack Studio 2.0 problem, the cause seems to be a Downloader Trojan on your computer (maybe TrojanDownloader.Win32.Dia.a) which places programs on your machine, removing and/or damaging autoexec.nt, config.nt and command.com.
For more information go Here. You will find a free download to restore the three files, unzip and copy them to C:\WINDOWS\system32. You should also look into the recommended actions.
*15.07.2015: Moderator notice: This is a 16-bit program that will not run on 64-bit versions of Windows.